5DRB
Crystal structure of WNK1 in complex with WNK463
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.642, 57.670, 65.532 |
| Unit cell angles | 90.00, 89.90, 90.00 |
Refinement procedure
| Resolution | 65.530 - 1.650 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.24050 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.530 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.403 | |
| Number of reflections | 34584 | |
| <I/σ(I)> | 21.8 | 2.2 |
| Completeness [%] | 99.4 | 98.1 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 298 | 100 mM Tris, pH 8.0, 16% PEG550 MME, 4% PEG3350 |
