5DQO
Crystal structure of Y347F mutant of human primase p58 iron-sulfur cluster domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.398, 60.398, 246.729 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.140 - 2.300 |
| R-factor | 0.25174 |
| Rwork | 0.251 |
| R-free | 0.27490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l9q |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 82.243 | 50.000 | 2.530 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.480 |
| Rmerge | 0.058 | 0.033 | 0.493 |
| Number of reflections | 42683 | ||
| <I/σ(I)> | 12.8 | 2.1 | |
| Completeness [%] | 92.5 | 99.1 | 89.6 |
| Redundancy | 4.5 | 5.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 300 mM lithium sulfate, 100 mM Tris, pH 8.5, 20% PEG3350 |
