5DQ0
Structure of human neuropilin-2 b1 domain with novel and unique zinc binding site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2012-06-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 99.390, 105.270, 45.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.130 - 1.800 |
R-factor | 0.1791 |
Rwork | 0.177 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qqj |
RMSD bond length | 0.016 |
RMSD bond angle | 1.753 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.130 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.052 | 0.230 |
Number of reflections | 22736 | |
<I/σ(I)> | 16.5 | |
Completeness [%] | 99.9 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289.15 | 25 % PEG 550 MME, 0.1 M MES pH 6.5, 0.01 M ZnSO4 |