5DPT

Crystal structure of PLEKHM1 LIR-fused human GABARAPL1_2-117

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 32 2 1
Unit cell lengths	84.791, 84.791, 105.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.826 - 2.900
R-factor	0.2162
Rwork	0.214
R-free	0.25950
RMSD bond length	0.003
RMSD bond angle	0.778
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.826	3.030
High resolution limit [Å]	2.900	2.900
Number of reflections	10119
<I/σ(I)>	10.6
Completeness [%]	99.9	99.5
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	278	20% w/v Polyethylene glycol 3,350, 0.2 M NaCl, 8% MPD pH 7.2