5DPM
Crystal structure of UbiG mutant in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-04-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.842, 39.326, 40.112 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 39.997 - 2.100 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kdc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 12887 | |
| <I/σ(I)> | 11.9 | 3 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 287 | 0.1 M citric acid pH 5.0, 20% (v/v) 2-Methyl-2,4-pentanediol |
