5DOB
Crystal structure of the Human Cytomegalovirus Nuclear Egress Complex (NEC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.254, 33.978, 92.995 |
Unit cell angles | 90.00, 121.76, 90.00 |
Refinement procedure
Resolution | 79.070 - 2.470 |
R-factor | 0.25644 |
Rwork | 0.254 |
R-free | 0.29920 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.019 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.080 | |
High resolution limit [Å] | 2.470 | 2.470 |
Number of reflections | 13720 | |
<I/σ(I)> | 14.47 | |
Completeness [%] | 97.5 | 88.9 |
Redundancy | 3.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 298 | 0.1 M Tris, 0.1 M NaCl, 10-15% PEG 3350 (w/v), 0.3-0.5 M CaCl2 |