5DNM
Nucleosome core particle containing adducts of ruthenium(II)-toluene PTA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2011-07-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.680, 109.820, 182.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.612 - 2.810 |
| R-factor | 0.20892 |
| Rwork | 0.208 |
| R-free | 0.24052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mnn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.499 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.780 | 2.960 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.059 | 0.501 |
| Number of reflections | 50790 | |
| <I/σ(I)> | 15.5 | 2.3 |
| Completeness [%] | 96.2 | 78.2 |
| Redundancy | 6.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40 mM MnCl2, 30 mM KCl, 20 mM K-Cacodylate |
