5DMR
Crystal Structure of C-terminal domain of mouse eRF1 in complex with RNase H domain of RT of Moloney Murine Leukemia Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.630, 63.862, 60.431 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 59.990 - 2.800 |
| R-factor | 0.2107 |
| Rwork | 0.207 |
| R-free | 0.28032 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2HB5 and 1DT9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.577 |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.620 | |
| Number of reflections | 8469 | |
| <I/σ(I)> | 23.6 | 3.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 0.1 M Tris pH 8.5, 18 % ammonium dihydrogen phosphate |
