Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

5DMI

Structure of the extracellular domain of the CD40 in complex with CHI220 FAB

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCLSI BEAMLINE 08ID-1
Synchrotron siteCLSI
Beamline08ID-1
Temperature [K]100
Detector technologyCCD
Collection date2010-01-29
DetectorRAYONIX MX-225
Wavelength(s)0.97949
Spacegroup nameP 42 21 2
Unit cell lengths201.152, 201.152, 63.809
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution32.640 - 3.690
R-factor0.1702
Rwork0.168
R-free0.20700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)CD40 ENSEMBLE FROM 1JMA 2uwi 2aw2 1ncf 1tnr 2hev 2HEY; VL FROM 3D85; VH FROM 2ZPK; CL:CH1 FROM 2O5X
RMSD bond length0.010
RMSD bond angle1.390
Data reduction softwareXDS
Data scaling softwareSCALA
Phasing softwarePHASER
Refinement softwareBUSTER (2.11.6)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]201.1503.890
High resolution limit [Å]3.6903.690
Rmerge0.495
Number of reflections14668
<I/σ(I)>13.26.8
Completeness [%]100.0100
Redundancy15.916.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.629380mM sodiym acetate, pH 4.6, 1.6 M ammonium sulfate, 20% (v/v) glycerol

227344

PDB entries from 2024-11-13

PDB statisticsPDBj update infoContact PDBjnumon