5DMI
Structure of the extracellular domain of the CD40 in complex with CHI220 FAB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 201.152, 201.152, 63.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.640 - 3.690 |
R-factor | 0.1702 |
Rwork | 0.168 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CD40 ENSEMBLE FROM 1JMA 2uwi 2aw2 1ncf 1tnr 2hev 2HEY; VL FROM 3D85; VH FROM 2ZPK; CL:CH1 FROM 2O5X |
RMSD bond length | 0.010 |
RMSD bond angle | 1.390 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 201.150 | 3.890 |
High resolution limit [Å] | 3.690 | 3.690 |
Rmerge | 0.495 | |
Number of reflections | 14668 | |
<I/σ(I)> | 13.2 | 6.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.9 | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 80mM sodiym acetate, pH 4.6, 1.6 M ammonium sulfate, 20% (v/v) glycerol |