5DLK
The crystal structure of CT mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.640, 221.678, 99.780 |
Unit cell angles | 90.00, 127.90, 90.00 |
Refinement procedure
Resolution | 41.906 - 1.800 |
R-factor | 0.1944 |
Rwork | 0.193 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dij |
RMSD bond length | 0.007 |
RMSD bond angle | 1.096 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.880 | |
Number of reflections | 256485 | |
<I/σ(I)> | 22.4 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Sodium malnate, Bis-Tris propane, PEG 3350 |