5DLK
The crystal structure of CT mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.640, 221.678, 99.780 |
| Unit cell angles | 90.00, 127.90, 90.00 |
Refinement procedure
| Resolution | 41.906 - 1.800 |
| R-factor | 0.1944 |
| Rwork | 0.193 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dij |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.096 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.880 | |
| Number of reflections | 256485 | |
| <I/σ(I)> | 22.4 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Sodium malnate, Bis-Tris propane, PEG 3350 |
