5DKA
A C2HC zinc finger is essential for the activity of the RING ubiquitin ligase RNF125
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.670, 52.320, 73.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.710 - 1.550 |
R-factor | 0.2113 |
Rwork | 0.210 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hcs |
RMSD bond length | 0.021 |
RMSD bond angle | 2.142 |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | XSCALE (November 3, 2014) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.590 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.055 | 0.799 |
Number of reflections | 28019 | |
<I/σ(I)> | 10.2 | 1.3 |
Completeness [%] | 94.0 | 94.7 |
Redundancy | 2.9 | 2.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 273 | 0.1 M HEPES, 10% PEG 8K |