5DJD

Fc Heterodimer Design 5.1 T366V + Y407F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2013-10-07
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	60.385, 67.435, 79.029
Unit cell angles	90.00, 105.29, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.22264
Rwork	0.220
R-free	0.27571
RMSD bond length	0.007
RMSD bond angle	1.261
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	67.435	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.071	0.508
Rmeas	0.170
Rpim	0.088	0.043	0.332
Total number of observations	99778	2830	14869
Number of reflections	26867
<I/σ(I)>	5.1	8.6	2
Completeness [%]	97.9	90.1	99.9
Redundancy	3.7	3.4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	100mM Imidazole pH 7 + 20% PEG 6000