5DJC

Fc Heterodimer Design 8.1 L368V/Y407A + T366V/K409F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2013-10-02
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 1
Unit cell lengths	60.242, 66.858, 72.771
Unit cell angles	85.17, 80.71, 89.69

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.23192
Rwork	0.229
R-free	0.28465
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.177
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	71.559	2.210
High resolution limit [Å]	2.100	6.640	2.100
Rmerge		0.044	0.264
Rmeas	0.092
Rpim	0.065	0.023	0.630
Total number of observations	123662	3491	17079
Number of reflections	62804
<I/σ(I)>	6.3	16.5	1.3
Completeness [%]	96.4	88.1	95.2
Redundancy	2	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	17% PEG 3350 + 100mM Ammonium Iodide