5DJA
Fc Heterodimer Design 9.1 Y407M + T366I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2013-10-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.657, 67.201, 78.612 |
| Unit cell angles | 90.00, 105.07, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.21043 |
| Rwork | 0.207 |
| R-free | 0.28097 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.316 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 75.907 | 3.160 |
| High resolution limit [Å] | 2.900 | 9.490 | 3.000 |
| Rmerge | 0.085 | 0.420 | |
| Rmeas | 0.241 | ||
| Rpim | 0.125 | 0.056 | 0.273 |
| Total number of observations | 43659 | 1323 | 6735 |
| Number of reflections | 13191 | ||
| <I/σ(I)> | 4.3 | 6.8 | 1.9 |
| Completeness [%] | 95.8 | 92.6 | 99.8 |
| Redundancy | 3.7 | 3.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 17% PEG 3350 + 100mM Ammonium Iodide |
