5DI8
Fc Knob-Hole Heterodimer T366W + T366S/L368A/Y407V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2013-05-31 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.297, 66.835, 79.214 |
Unit cell angles | 90.00, 104.97, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.22666 |
Rwork | 0.224 |
R-free | 0.27511 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dn2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.551 |
Data scaling software | SCALA (3.3.20) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 66.835 | 2.000 |
High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
Rmerge | 0.045 | 0.350 | |
Rmeas | 0.088 | ||
Rpim | 0.045 | 0.027 | 0.248 |
Total number of observations | 173293 | 4765 | 24277 |
Number of reflections | 46181 | ||
<I/σ(I)> | 9.5 | 16.1 | 3 |
Completeness [%] | 96.2 | 86 | 95.4 |
Redundancy | 3.8 | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | 100mM Hepes pH 7.5 + 2% Isopropanol + 18% PEG 8K + 100mM Sodium Acetate |