5DI0
Crystal structure of Dln1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.111, 95.100, 147.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.600 - 1.700 |
R-factor | 0.17407 |
Rwork | 0.172 |
R-free | 0.20887 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zno |
RMSD bond length | 0.010 |
RMSD bond angle | 1.453 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.459 | |
Number of reflections | 87589 | |
<I/σ(I)> | 14.8 | 2.9 |
Completeness [%] | 98.6 | 98.7 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4 | 289 | 12% PEG 4K,0.2M NH4Ac, 0.1M NaAc pH 4.0 |