5DGY
Crystal structure of rhodopsin bound to visual arrestin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.484, 107.258, 460.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.978 - 7.700 |
| R-factor | 0.2877 |
| Rwork | 0.285 |
| R-free | 0.33510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zwj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.021 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 8.100 |
| High resolution limit [Å] | 7.700 | 7.700 |
| Rmerge | 0.314 | 1.188 |
| Number of reflections | 6795 | |
| <I/σ(I)> | 3.79 | 1.08 |
| Completeness [%] | 97.5 | 0.908 |
| Redundancy | 4.68 | 4.12 |
| CC(1/2) | 1.000 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 293 | PEG400 |
