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5DGV

Complex of yeast 80S ribosome with hypusine-containing/non-modified eIF5A and/or a peptidyl-tRNA analog

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSOLEIL BEAMLINE PROXIMA 1
Synchrotron siteSOLEIL
BeamlinePROXIMA 1
Temperature [K]100
Detector technologyPIXEL
Collection date2014-03-15
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.14271
Spacegroup nameP 1 21 1
Unit cell lengths437.140, 287.910, 303.850
Unit cell angles90.00, 98.76, 90.00
Refinement procedure
Resolution149.047 - 3.100
R-factor0.2389
Rwork0.238
R-free0.28400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4v88
RMSD bond length0.006
RMSD bond angle1.018
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHENIX
Refinement softwarePHENIX
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]149.0473.200
High resolution limit [Å]3.100125.0003.100
Rmerge0.5570.0561.379
Rmeas0.5940.0671.575
Total number of observations6878841
Number of reflections133835712121237
<I/σ(I)>7.977.861.34
Completeness [%]99.944.4100
Redundancy5.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7277.15Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000

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