5DGV

Complex of yeast 80S ribosome with hypusine-containing/non-modified eIF5A and/or a peptidyl-tRNA analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.14271
Spacegroup name	P 1 21 1
Unit cell lengths	437.140, 287.910, 303.850
Unit cell angles	90.00, 98.76, 90.00

Refinement procedure

Resolution	149.047 - 3.100
R-factor	0.2389
Rwork	0.238
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v88
RMSD bond length	0.006
RMSD bond angle	1.018
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	149.047		3.200
High resolution limit [Å]	3.100	125.000	3.100
Rmerge	0.557	0.056	1.379
Rmeas	0.594	0.067	1.575
Total number of observations	6878841
Number of reflections	1338357	12	121237
<I/σ(I)>	7.97	7.86	1.34
Completeness [%]	99.9	44.4	100
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000