5DFH
Human APE1 mismatch product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-13 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 44.434, 61.603, 72.277 |
Unit cell angles | 83.86, 78.76, 88.19 |
Refinement procedure
Resolution | 26.260 - 1.949 |
R-factor | 0.1804 |
Rwork | 0.179 |
R-free | 0.21870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iem |
RMSD bond length | 0.007 |
RMSD bond angle | 1.066 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.949 |
Rmerge | 0.058 |
<I/σ(I)> | 20.8 |
Completeness [%] | 96.1 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 10% Peg20000, 100 mM sodium citrate, 200 mM MgCl |