5DEU
Crystal structure of TET2-5hmC complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97931 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.262, 87.522, 260.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.262 - 1.801 |
R-factor | 0.1785 |
Rwork | 0.177 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nm6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.094 | 0.079 | 0.828 |
Total number of observations | 487032 | ||
Number of reflections | 50752 | ||
<I/σ(I)> | 16.3 | ||
Completeness [%] | 98.0 | 95.7 | 88.6 |
Redundancy | 9.6 | 9.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 277 | 26% PEGMME2000,0.1M MES6.4 |