5DEU

Crystal structure of TET2-5hmC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	C 2 2 21
Unit cell lengths	48.262, 87.522, 260.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.262 - 1.801
R-factor	0.1785
Rwork	0.177
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nm6
RMSD bond length	0.006
RMSD bond angle	1.150
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.094	0.079	0.828
Total number of observations	487032
Number of reflections	50752
<I/σ(I)>	16.3
Completeness [%]	98.0	95.7	88.6
Redundancy	9.6	9.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	277	26% PEGMME2000,0.1M MES6.4