5DER
RNA oligonucleotide containing (R)-C5'-ME-2'F U
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-30 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 44.116, 44.116, 86.927 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.340 - 1.800 |
R-factor | 0.248 |
Rwork | 0.257 |
R-free | 0.27400 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.856 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.340 | 1.890 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.099 | 0.406 |
Number of reflections | 15381 | |
<I/σ(I)> | 9.57 | 1.77 |
Completeness [%] | 99.6 | 97.7 |
Redundancy | 4.9 | 2.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 40 mM sodium cacodylate, 20 mM hexamine Co(III) chloride, 12 mM NaCl, 80 mM KCl, reservoir 40% MPD |