5DEP
Structure of Pseudomonas aeruginosa LpxA in complex with UDP-GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1270 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.038, 83.559, 222.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 111.210 - 2.160 |
R-factor | 0.18 |
Rwork | 0.177 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.558 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 111.210 |
High resolution limit [Å] | 2.060 |
Number of reflections | 79955 |
<I/σ(I)> | 13.128 |
Completeness [%] | 98.4 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M calcium acetate, 0.1M imidazole pH8.0, 20% PEG 1000, 50mM UDP-GlcNAc |