5DED
Crystal structure of the small alarmone synthethase 1 from Bacillus subtilis bound to its product pppGpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 197.869, 113.492, 139.848 |
| Unit cell angles | 90.00, 127.17, 90.00 |
Refinement procedure
| Resolution | 50.567 - 2.942 |
| R-factor | 0.1838 |
| Rwork | 0.180 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dec |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.600 | |
| High resolution limit [Å] | 2.940 | |
| Rmerge | 0.103 | 0.273 |
| Number of reflections | 51454 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 96.1 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Citric acid, pH 4.0, 5% (w/v) PEG 6000 |
