5DE8
Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-28 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.10528 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.650, 130.600, 36.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.355 - 3.100 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.22730 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.049 | 0.242 |
| Number of reflections | 9586 | |
| <I/σ(I)> | 26.7 | 7.45 |
| Completeness [%] | 98.9 | 98.8 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate pH 5.6, 0.2 M ammonium acetate, 18% PEG 4000. Incubated with 10 mM iridium hexammine for heavy atom derivation. |
