5DE5
Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-23 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.1053 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.060, 130.010, 36.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.218 - 3.001 |
| R-factor | 0.1958 |
| Rwork | 0.195 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.846 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.011 | 0.665 |
| Number of reflections | 10504 | |
| <I/σ(I)> | 12.9 | 3.3 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M Na-citrate pH 5.6, 0.2 M ammonium acetate, 18% PEG 4000 |
