5DDY

Binary complex of human Polymerase lambda with dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	95
Detector technology	CCD
Collection date	2012-10-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	206.015, 206.015, 114.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.658 - 3.360
R-factor	0.2637
Rwork	0.262
R-free	0.29690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.002
RMSD bond angle	0.630
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.490
High resolution limit [Å]	3.360	7.230	3.370
Rmerge	0.072	0.034	0.825
Total number of observations	390025
Number of reflections	35439
<I/σ(I)>	16.3
Completeness [%]	99.8	99	99.1
Redundancy	11	10	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M HEPES, 3.0M Sodium Chloride