5DDY
Binary complex of human Polymerase lambda with dCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 206.015, 206.015, 114.266 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.658 - 3.360 |
R-factor | 0.2637 |
Rwork | 0.262 |
R-free | 0.29690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xsl |
RMSD bond length | 0.002 |
RMSD bond angle | 0.630 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.490 |
High resolution limit [Å] | 3.360 | 7.230 | 3.370 |
Rmerge | 0.072 | 0.034 | 0.825 |
Total number of observations | 390025 | ||
Number of reflections | 35439 | ||
<I/σ(I)> | 16.3 | ||
Completeness [%] | 99.8 | 99 | 99.1 |
Redundancy | 11 | 10 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES, 3.0M Sodium Chloride |