5DDR
L-glutamine riboswitch bound with L-glutamine soaked with Cs+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-04 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.967, 86.882, 62.218 |
| Unit cell angles | 90.00, 102.61, 90.00 |
Refinement procedure
| Resolution | 29.368 - 2.605 |
| R-factor | 0.2175 |
| Rwork | 0.212 |
| R-free | 0.27050 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| <I/σ(I)> | 13.4 |
| Completeness [%] | 93.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | .1 M HEPES-sodium, pH 7.0, 40% (v/v) 2-methyl-2,4-pentanediol, 20 mM CsCl |
