5DCL
Structure of a lantibiotic response regulator: N terminal domain of the nisin resistance regulator NsrR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0791 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.050, 107.130, 39.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.740 - 1.410 |
R-factor | 0.1967 |
Rwork | 0.196 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.091 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 1.410 |
Rmerge | 0.058 |
Number of reflections | 440765 |
<I/σ(I)> | 21.05 |
Completeness [%] | 98.7 |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 0.1 M SPG buffer pH 8.0 and 25% (w/v) PEG 1500 |