Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.148 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 438.230, 289.330, 305.470 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 190.477 - 3.250 |
| R-factor | 0.2533 |
| Rwork | 0.252 |
| R-free | 0.30090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v88 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.024 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 190.477 | 3.300 | |
| High resolution limit [Å] | 3.250 | 175.000 | 3.250 |
| Rmerge | 0.411 | 1.530 | 2.179 |
| Rmeas | 0.434 | 1.688 | 2.356 |
| Total number of observations | 9652670 | ||
| Number of reflections | 1176614 | 3 | 52321 |
| <I/σ(I)> | 6.38 | 1.16 | 0.98 |
| Completeness [%] | 100.0 | 30 | 100 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000 |
