5DBR
Ca2+ CaM with human cardiac Na+ channel (NaV1.5) inactivation gate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-31 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 58.086, 58.086, 102.638 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.300 - 2.250 |
R-factor | 0.2236 |
Rwork | 0.220 |
R-free | 0.28420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4djc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.913 |
Data reduction software | PROTEUM PLUS |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.300 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.090 | 0.446 |
Number of reflections | 9401 | |
<I/σ(I)> | 18.4 | 4.1 |
Completeness [%] | 99.6 | 100 |
Redundancy | 15.6 | 10.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.2M sodium malonate pH 4.0, 20% w/v PEG 3350 |