5DB8
Structure of human DNA polymerase beta Host-Guest complex with the N7MG base paired with a dA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.149, 78.904, 54.751 |
| Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
| Resolution | 19.726 - 2.547 |
| R-factor | 0.1838 |
| Rwork | 0.180 |
| R-free | 0.25770 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.318 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.540 |
| Number of reflections | 14558 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 100.0 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole |
