5DB6
Structure of human DNA polymerase beta Host-Guest complex with the N7MG base paired with a dC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97648 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.138, 78.828, 54.764 |
Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
Resolution | 19.707 - 2.830 |
R-factor | 0.1889 |
Rwork | 0.185 |
R-free | 0.26590 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.834 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.830 |
Number of reflections | 10562 |
<I/σ(I)> | 9.4 |
Completeness [%] | 100.0 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole |