5DAH

Crystal structure of PZP domain of human AF10 protein fused with Histone H3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	42.905, 79.995, 59.287
Unit cell angles	90.00, 102.01, 90.00

Refinement procedure

Resolution	46.951 - 2.611
R-factor	0.1824
Rwork	0.176
R-free	0.23970
RMSD bond length	0.009
RMSD bond angle	1.116
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.000
High resolution limit [Å]	2.610
Number of reflections	11663
<I/σ(I)>	10.7
Completeness [%]	97.1
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium citrate tribasic dehydrate, pH 5.6, 0.2 M potassium sodium tartrate tetrahydrate, 1.8 M ammonium sulfate