5DA6
Atomic resolution crystal structure of double-stranded RNA 32 base pairs long determined from random starting phases angles in the presence of pseudo translational symmetry using the direct methods program SIR2014.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 42.890, 42.890, 266.936 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.789 - 1.050 |
| R-factor | 0.1301 |
| Rwork | 0.129 |
| R-free | 0.15680 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.038 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | Sir2014 |
| Refinement software | PHENIX (1.10_2142) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.790 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.053 | 0.369 |
| Number of reflections | 44942 | |
| <I/σ(I)> | 20.1 | 3.8 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Lithium sulfate, magnesium chloride, cacodylate |
