5D9I
SV40 Large T antigen origin binding domain bound to artificial DNA fork
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0809 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.192, 63.393, 64.891 |
Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.18174 |
Rwork | 0.180 |
R-free | 0.22257 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fuf |
RMSD bond length | 0.017 |
RMSD bond angle | 1.709 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0129) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 37425 | |
<I/σ(I)> | 15.9 | |
Completeness [%] | 88.8 | 54.7 |
Redundancy | 7.3 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Tris, 300 mM NH4Cl, 28% polyethylene glycol [PEG] 4000, 20 mM CaCl2 |