5D8C
Crystal structure of HiNmlR, a MerR family regulator lacking the sensor domain, bound to promoter DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.030, 111.820, 103.170 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 37.450 - 2.250 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gpv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.450 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.675 | |
| Number of reflections | 22051 | |
| <I/σ(I)> | 1.1 | |
| Completeness [%] | 14.3 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% w/v PEG 3,350; 0.4 M sodium nitrate; 0.1 M Bis-Tris propane; 2 mM TCEP |
