5D7P
Crystal structure of human Sirt2 in complex with ADPR and EX-243
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.496, 77.578, 114.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 1.760 |
| R-factor | 0.19182 |
| Rwork | 0.190 |
| R-free | 0.22444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d7o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.340 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.410 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.080 | 1.136 |
| Number of reflections | 66454 | |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 97.8 | |
| Redundancy | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.75 | 293 | 18 % (w/v) PEG 10000, 0.1 M Bis-Tris |
