5D7G
Structure of human ATG5 E122D-ATG16L1 complex at 3.0 Angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 217.114, 84.478, 151.849 |
Unit cell angles | 90.00, 133.81, 90.00 |
Refinement procedure
Resolution | 49.535 - 3.000 |
R-factor | 0.2003 |
Rwork | 0.198 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tq0 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.994 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.120 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.057 | 0.645 |
Number of reflections | 39496 | |
<I/σ(I)> | 14.3 | 2.3 |
Completeness [%] | 98.9 | 99.3 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Micro crystal: 37.5 mM MES, pH 5.2 to pH 5.8, 0.2 M sodium tartrate, and 11 to 13% polyethylene glycol 3350; Micor seeding condition:40 mM MES, pH 5.5, 0.2M sodium tartrate, 8.5% PEG3350, 10 mM DTT |