5D68
Crystal structure of KRIT1 ARD-FERM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.734, 80.242, 213.145 |
| Unit cell angles | 90.00, 91.69, 90.00 |
Refinement procedure
| Resolution | 49.369 - 2.908 |
| R-factor | 0.2086 |
| Rwork | 0.207 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 4DXA & 4HB5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1760) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.192 | 0.899 |
| Number of reflections | 46892 | |
| <I/σ(I)> | 7.5 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.05 M HEPES, pH 7.3, 8% ethylene glycol, 8% PEG8000 |
