5D5B
In meso X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.03321 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.000, 170.000, 40.500 |
Unit cell angles | 90.00, 106.25, 90.00 |
Refinement procedure
Resolution | 49.618 - 3.800 |
R-factor | 0.2416 |
Rwork | 0.240 |
R-free | 0.27440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rh1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.144 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.900 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 6835 | |
<I/σ(I)> | 3.76 | 0.96 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 6.5 | 4.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol |