5D4K
Crystal structure of the human polymeric Ig receptor (pIgR) ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.258, 242.434, 63.046 |
| Unit cell angles | 90.00, 114.89, 90.00 |
Refinement procedure
| Resolution | 36.984 - 2.599 |
| R-factor | 0.2032 |
| Rwork | 0.201 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xed |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.623 | 39.623 | 2.740 |
| High resolution limit [Å] | 2.599 | 8.220 | 2.600 |
| Rmerge | 0.021 | 0.559 | |
| Rmeas | 0.070 | ||
| Rpim | 0.039 | 0.015 | 0.373 |
| Total number of observations | 145998 | 4461 | 21108 |
| Number of reflections | 49242 | ||
| <I/σ(I)> | 13.7 | 37.4 | 2.2 |
| Completeness [%] | 96.5 | 92.8 | 95.6 |
| Redundancy | 3 | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.2 M ammonium sulfate, 0.1M BisTris pH 5.5, 31% Polyethylene glycol 3350 |
