5D3Q
Dynamin 1 GTPase-BSE fusion dimer complexed with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 |
Unit cell lengths | 44.220, 49.960, 82.520 |
Unit cell angles | 99.40, 87.18, 105.18 |
Refinement procedure
Resolution | 47.626 - 1.700 |
R-factor | 0.1744 |
Rwork | 0.173 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x2e |
RMSD bond length | 0.015 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.626 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.118 | 0.245 |
Number of reflections | 72213 | |
<I/σ(I)> | 10.75 | 6.7 |
Completeness [%] | 97.6 | 96.1 |
Redundancy | 4.63 | 3.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Before crystallization set-ups, GG1 at a concentration of 10 mg/ml was supplemented with 2 mM GDP and 2 mM MgCl2 and incubated for 1 h on ice. GG1GDP was crystallized by hanging drop vapour diffusion mixing 1.5 ul protein solution with 1.5 ul reservoir solution containing 0.1 M Tris (pH 8.0), 26 % PEG 3350 and 0.2 M NaSCN. The drops were equilibrated against 700 ul of reservoir. |