5D3M
Folate ECF transporter: AMPPNP bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972423 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.070, 97.260, 105.450 |
| Unit cell angles | 84.67, 64.78, 62.59 |
Refinement procedure
| Resolution | 43.862 - 3.303 |
| R-factor | 0.2523 |
| Rwork | 0.250 |
| R-free | 0.28920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hzu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.404 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.026 | 0.867 |
| Number of reflections | 40590 | |
| <I/σ(I)> | 12 | 0.5 |
| Completeness [%] | 94.4 | 94.4 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 50mM TRIS, 17% PEG 350 MME, 2% NG, 10mM spermidine, 10mM MgCl2, 10mM AMP-PNP |
