5D2M

Complex between human SUMO2-RANGAP1, UBC9 and ZNF451

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	78.240, 115.060, 130.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.349 - 2.400
R-factor	0.196
Rwork	0.195
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uio
RMSD bond length	0.003
RMSD bond angle	0.743
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.350	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.120	0.514
Number of reflections	46766
<I/σ(I)>	10.94	3.83
Completeness [%]	99.6	96.6
Redundancy	7.3	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	6 % (w/v) PEG 8000, 0.2 M ammonium citrate and 0.1 M HEPES pH 7.5