5D28
Complex of GM-CSF/IL-2 inhibition factor with Granulocyte-macrophage colony-stimulating factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.700, 105.600, 73.490 |
Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.960 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 1.120 | |
Number of reflections | 25798 | |
<I/σ(I)> | 20.39 | 1.77 |
Completeness [%] | 98.2 | 95.7 |
Redundancy | 6.9 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.15 M ammonium sulfate, 0.1 M MES pH 6.0, 24 % PEG 4000 |