5D22
Structure of ovine granulocyte-macrophage colony-stimulating factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.239 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.100, 77.020, 47.450 |
Unit cell angles | 90.00, 111.54, 90.00 |
Refinement procedure
Resolution | 38.510 - 1.994 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gmf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.068 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.510 | 2.110 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 18252 | |
<I/σ(I)> | 8.17 | 2.12 |
Completeness [%] | 96.9 | 94 |
Redundancy | 3.28 | 3.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M sodium acetate trihydrate 0.1 M TRIS hydrochloride pH 8.5 30% w/v PEG 4000 |