5D1M
Crystal Structure of UbcH5B in Complex with the RING-U5BR Fragment of AO7 (P199A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.696, 47.415, 67.975 |
| Unit cell angles | 90.00, 118.32, 90.00 |
Refinement procedure
| Resolution | 30.398 - 1.581 |
| R-factor | 0.1891 |
| Rwork | 0.188 |
| R-free | 0.22310 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5d1k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
| Rmerge | 0.033 | 0.027 | 0.289 |
| Number of reflections | 38987 | ||
| <I/σ(I)> | 23 | 75.779 | 5.296 |
| Completeness [%] | 99.7 | 99.7 | 96.9 |
| Redundancy | 6.2 | 6.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20% PEG 10000, 8% Ethylene glycol, 0.1M HEPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
