5D1L
Crystal Structure of UbcH5B in Complex with the RING-U5BR Fragment of AO7 (Y165A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-05-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.595, 47.653, 67.816 |
| Unit cell angles | 90.00, 118.03, 90.00 |
Refinement procedure
| Resolution | 42.430 - 1.618 |
| R-factor | 0.1822 |
| Rwork | 0.182 |
| R-free | 0.19950 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5d1k |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 3.470 | 1.610 |
| Rmerge | 0.034 | 0.024 | 0.355 |
| Number of reflections | 35154 | ||
| <I/σ(I)> | 20 | ||
| Completeness [%] | 94.8 | 99.8 | 75.9 |
| Redundancy | 7.2 | 7.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 15% PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
