5D0U
Crystal structure of the RNA-helicase Prp43 from Chaetomium thermophilum bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 65 |
| Unit cell lengths | 152.211, 152.211, 92.766 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.940 - 2.919 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xau |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.843 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.940 | 2.992 |
| High resolution limit [Å] | 2.919 | 2.919 |
| Rmerge | 0.086 | |
| Number of reflections | 26545 | |
| <I/σ(I)> | 15.39 | |
| Completeness [%] | 99.3 | 97 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 8 % (w/v) Jeffamine M2070, 0.17 M Glycine, 16.7 % (v/v) DMSO, 10 mM Urea |
